Ethological dynamics in diorama environments

In this project, we will develop a visualization method to observe the behavior of protists at the cellular level in a highly fluctuating real environment "as it is".
If we can obtain microbial movement information with high temporal resolution without disturbing the biological information, it will lead to a better understanding of the dynamics of microbial suspension flow. In order to obtain more refined observation conditions (accuracy, resolution, and reproducibility) without affecting environmental information (temperature, density, concentration, etc.) and biological information (metabolism, absorption, degradation, movement, genetic information, etc.) by observation methods, analysis and analysis that take into account the constraints of observation conditions are necessary. By constructing a Diorama environments that awakens the abilities of protists, we aim to elucidate the microbial flow mechanisms in cell suspensions by using the latest measurement techniques for cell population movement and localization.

In the experiment on sperm, I will collaborate with Dr. Shiba of group A01 and Dr. Ishikawa of group B01-1. In the experiment on microalgae, I will collaborate with Dr. Shikada of Group A01 and Dr. Ishikawa of Group B01-1. We will construct diorama environments and visualization methods for other groups in the area, and build experimental systems and analysis methods to verify microbial flow mechanisms.
The team will support the production of Diorama environments in the entire research area.

In vivo live image of gut microbiota spatial temporal distribution in the zebrafish larval posterior intestine

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